Immobilization of alpha-naphthyl acetate esterase (ANAE) on k-carrageenan for potential biosensor application in pesticide detection

In this study, alpha-naphthyl acetate esterase (ANAE) extracted from Atta (wholemeal) wheat flour and grinded grains was immobilized on k-carrageenan beads that were amino-functionalized using 2% polyethyleneimine (PEI). ANAE is a plant enzyme wheat, where its activity can be inhibited by organophosphate (OP) pesticide for biosensor applications. SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) conducted to investigate molecular weight. in both free states have been investigated protein concentration, loading efficiency, reusability optimum temperature pH. The study showed the relative of remained higher than 90%, up 50 °C. Regardless source ANEA extraction, pH at 7–9. sources within 35–40 ℃, which slightly lower state. survived washing assay 11 cycles, regardless extraction.